The acquisition of human tissue is performed under the approval of the Bioethical Committee for Clinical Research of the University of Barcelona on October 9th 2015.
The human liver tissue is obtained by donation from two sources: patients undergoing planned liver resection surgeries due to primary or secondary tumors (living donors), and whole liver discarted for transplantation purposes. Nowadays, the program link 9 donor centers from Spain. Along with the tissue donation, demographic information and fully characterized donor clinic profile is obtained for use in bioanalytical processes.
Human tissue acquisition is performed according to Spanish and European legal requirements in order to ensure quality, safety and traceability of the generated material. Cytes Biotechnologies, S.L. complies with the following legislation and ethical principles:
Spanish Legislation: Real Decreto de establecimiento de tejidos 1301/2006, Ley de Protección de Datos 15/1999, Real Decreto 1716/2011 de Biobancos.
European Legislation: 2004/23/EC, 2006/17/EC, 2006/8/EC.
Ethical principles: Nüremberg Code (1946), Helsinki Declaration (1996) and Belmont Report (1978).
Cytes Biotechnologies, S.L. offers the hepatocytes in various formats:
Hepatocytes in suspension are preferable for short term studies whereas plated hepatocytes are more useful for long-term orientated studies.
Fresh human primary hepatocytes are used for several studies of cell metabolism:
Cytes Biotechnologies, S.L. offers cryopreserved hepatocytes well characterized and qualified for key applications in different formats:
• Cryopreserved Plateable Male Hepatocytes
• Cryopreserved Plateable Female Hepatocytes
• Cryopreserved Suspension Male Hepatocytes
• Cryopreserved Suspension Female Hepatocytes
• Rigorous QC standards.
• Extensive application based characterization
• Proprietary media for recovery, plating and maintenance
• Tested for yield, viability, and morphology
Human cryopreserved hepatocytes are stored in liquid nitrogen vapor (-150°C or colder). Cryopreserved human hepatocytes are used in the following studies: