Products - Human Hepatocytes

Human liver tissue sourcing network

The acquisition of human tissue is performed under the approval of the Bioethical Committee for Clinical Research of the University of Barcelona on October 9th 2015.

The human liver tissue is obtained by donation from two sources: patients undergoing planned liver resection surgeries (living donors), and whole liver discarted for transplantation purposes. Nowadays, the program link 9 donor centers from Spain. Along with the tissue donation, demographic information and fully characterized donor clinic profile is obtained for use in bioanalytical processes.

Human tissue acquisition is performed according to Spanish and European legal requirements in order to ensure quality, safety and traceability of the generated material. Cytes Biotechnologies, S.L. complies with the following legislation and ethical principles:

Spanish Legislation: Real Decreto de establecimiento de tejidos 1301/2006, Ley de Protección de Datos 15/1999, Real Decreto 1716/2011 de Biobancos.

European Legislation: 2004/23/EC, 2006/17/EC, 2006/8/EC.

Ethical principles: Nüremberg Code (1946), Helsinki Declaration (1996) and Belmont Report (1978).

Fresh Human Primary Hepatocytes

Cytes Biotechnologies, S.L. offers the hepatocytes in various formats:

Fresh Cold-Stored Suspension
Fresh 6/ 12 / 24 / 48 / 96 -well, No Overlay
Fresh 6/ 12 / 24 / 48 / 96, Overlay
Other formats: customised isolations, cells used for internal projects

Hepatocytes in suspension are preferable for short term studies whereas plated hepatocytes are more useful for long-term orientated studies.

Fresh human primary hepatocytes are used for several studies of cell metabolism:

  • Metabolic pathway identification and profiling
  • Metabolism of stable compounds
  • Determination of metabolic routes in metabolite formation
  • Assessment of effects on cell function

Also are used for Drug development studies, such as:

  • Drug-drug interaction
  • Evaluation of drug metabolism
  • Drug-mediated responses

And for Toxicity studies:
  • Toxicity studies
  • Hepatotoxicity
  • Detoxification effects
  • Uptake and efflux transporter activities

Other studies:
  • Assessment of potential enzyme induction or inhibition

Cryopreserved Human Hepatocytes

Cytes Biotechnologies, S.L. offers cryopreserved hepatocytes well characterized and qualified for key applications in different formats:

• Cryopreserved Plateable Male Hepatocytes

• Cryopreserved Plateable Female Hepatocytes

• Cryopreserved Suspension Male Hepatocytes

                         • Cryopreserved Suspension Female Hepatocytes

Product characteristics:

• Rigorous QC standards.
• Extensive application based characterization
• Proprietary media for recovery, plating and maintenance
• Tested for yield, viability, and morphology

Human cryopreserved hepatocytes are stored in liquid nitrogen vapor (-150°C or colder). Cryopreserved human hepatocytes are used in the following studies:

  • Acute Cytotoxicity Assessment.
  • Prediction of Hepatic Clearance.
  • Prediction of Fraction Metabolized (fm) close to in vivo.
  • Hepatocytes in suspension allow evaluation of donor-specific uptake transporter inhibition and CYP inhibition.
  • Evaluation of Repeat Drug Exposure.
  • Plateable cryopreserved hepatocytes allow the evaluation of chronic exposition to drugs by the measurement of inflammatory signals.
  • Study Donor dependent Drug Metabolism and Induction.
  • Plateable cryopreserved hepatocytes allow the evaluation of inter-individual variations in drug metabolism enzymes (DMEs) and response to inducers.